ACUTE TOXICITY AND DOSE RESPONSE STUDIES OF …

ACUTE TOXICITY AND DOSE RESPONSE STUDIES OF AQUEOUS AND ETHANOL EXTRACTS OF TRIPLOCHITON SCLEROXYLON K. SCHUM, (STERCULIACEAE). ... The acute toxicity and dose response studies of aqueous and 50% ethanol extracts of ... histological examination after 28 days of administration of plant extracts. The lethal dose (LD50) ...

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Determination of lethal dose (LD50) (acute toxicity testing) The acute toxicity of the plants extracts were tested on the mice using the method and calculation proposed by Lorke 1983[10].

STUDY THE PATHOLOGICAL EFFECTS OF CRUDE EXTRACT OF ...

2- Median lethal dose Graduated doses of Portulaca oleracea ethanolic extract were dissolved in 10 ml distill water and administered S/C as 0.1 ml for each 10 gm of animal body weight. The range was of S/C single doses used in the determination of LD50 of the extract was (5000- 9500) mg /kg B.W.

Phytochemical screening, proximate analysis, median lethal ...

mice were used for determination of median lethal dose of the aqueous and ethyl acetate extracts respectively. The LD 50 ... Preparation of extract: The plant parts (seeds) were thoroughly washed with tap water, dried on filter papers and air dried under open shade for more than a month.

Histopathological Responses of the Heart, Liver and Kidney ...

The determination of the LD 50 of plant extract and the venom was done using the method of Lorke (1983). ... 50 was determined to form a basis of dosage for subsequent assays employing sublethal doses of plant extract and venom. The LD 50 of C. portoricensis which ... The geometric mean of the non-lethal dose and the lowest lethal dose was ...

Laboratory determination of efficacy of indigenous plant ...

Laboratory determination of efficacy of indigenous plant extracts for parasites control. Authors; Authors and affiliations; ... Reddy PJ, Krishna D, Murthy US, Jamil K (1992) A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. CABIOS 8:209–213 PubMed Google Scholar.

A PILOT STUDY ON THE DETERMINATION OF ANTIOXIDANT ...

the lethal dosage of fruit extract of . Terminalia chebula, a traditional medicinal plant widely used for various ailments. 50 . Results: Phytochemical analysis of revealed. T. Chebula the quantit of alkaloidsy (2.85mg/g), phenols (1.72 mg/g), terpenoids (1.06 mg/g) and flavonoids (0.560 mg/g).

Establishment of Mean Lethal Dose (LD 50) of Crude ...

Establishment of Mean Lethal Dose (LD 50) of Crude Ethanolic Extract (CEE) Mixture of Tinospora Rumphii Boerl, Chrysophyllum Cainito Linn. and Mimosa Pudica Linn ... regardless of the concentrations, the extracts were highly effective [5]. The efficacy of the plant solvent extract was based on the formula of categorization of anthelmintic ...

Oleander Uses, Benefits & Dosage - Drugs.com

The oleander plant contains a number of related cardiac glycosides similar in activity to digitalis. ... N. oleander extract displayed a dose-dependent inhibitory effect against the growth of lung carcinoma cells in rats. ... Toloudi M, Chatziioannou M, Papasotiriou I. Determination of efficacy of Anvirzel in 37 established cancer cell ...

Obtaining the Minimum Lethal Dose against Fasciola ...

Looking for an alternative control for this disease the present study was aimed at determining the hexane anti-Fasciola hepatica in the in vitro effect of some plant extracts and the minimum lethal dose of the mentioned extracts.

Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug ...

arithmetic method of Karber [15] was used for the determination of LD50. LD50 LD100 = −∑ ×(a bn) n = total number of animal in a group. a = the difference between two successive doses of administered extract/substance. b = the average number of dead animals in two successive doses. LD100 = Lethal dose causing the death of all test ...

Calendula Uses, Benefits & Dosage - Drugs.com

Calendula plant contains a number of oleanolic acid ... in the hepatic parenchyma were also noted.59 Saponin extracts of C. officinalis are not mutagenic.60 The median lethal dose of calendula flower extract in rats was more ... Dalla Via J, Guerriero A. Simultaneous quantitative determination of eight triterpenoid monoesters from flowers ...

EVALUATION OF THE LETHAL DOSE OF THE METHANOL …

prejudice to the toxic effects of plant extracts of which the leaf of mangrove is one, the present investigation aims to determine the mean lethal dose (LD 50 ... toxicity studies and determination 0f median lethal dose.Current Science 93(7): 1-4. Aliu YO, Nwude N. Veterinary Pharmacology and Toxicology Experiments. A.B.U. Press, Zaria,

Oral Administration of Aqueous Leaf Extract of Ocimum ...

The effects of oral administration of aqueous leaf extract of Ocimum gratissimum (OG) on food, water intake ... Oral Administration of Aqueous Leaf Extract of Ocimum Gratissimum Ameliorates Polyphagia, ... The median lethal dose (LD. 50) of the plant extract was determined by method of 2]Lorke (1983)[1 using 35 rats ...

Brine shrimp lethality and acute oral toxicity studies on ...

Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. ... (LC 50) and Median lethal dosage (LD 50) of Swietenia mahagoni crude methanolic ... It is suggested that the plant extract has a high amount of bioactive substances and may contain compounds that possess cytotoxicity effects.

lethal dose determination - amarcord.co.za

determination of lethal dose of plant extract . fulltext pdf The Journal of Microbiology, Biotechnology and Food . the Range test for determination of LD50 (Guneshwor and Bhagirath, 2005). The methanol and n >>Advisory. Ethyl Methane Sulphonate (EMS) Induced .

determination of lethal dose of plant extract

determination of lethal dose of plant extract 77 questions in Lethal Dose 50 | Science topic - ResearchGate. Median lethal dose (LD50) - The median lethal dose, LD50 (abbreviation for "lethal dose,50%") is the dose of pathogen required to kill half the members of a tested population.

Toxicological evaluation of methanol leaves extract of ...

LD50 determination A lethal dose for fifty percent of the mice (LD50) for methanol leaf extract was determined using a total number of 90 Swiss Albino mice that were divided into 9 groups of 10 mice. Eight groups of mice were ad-ministered with methanol leaf extract of the plant at doses from 1250mg/kg to 2750mg/kg in 250mg/kg

Antimicrobial activity and the median lethal dose - IASJ

for determination the ranges of lethal doses that used in acute toxicity study, two mice for each selected dose of extract. The selected doses were 1400 mg/kg B.W, and 1800 mg/Kg B.W according to the lethal outcome (death one or two mice) . The range of acute toxicity doses have been selected. b. Estimation of (LD 50

Toxicity Studies of the Crude Aqueous Leaves Extracts of ...

Acute toxicity study (Determination of LD 50) The median lethal dose (LD 50) of the plant extract was determined by method of Lorke (1983) [27] using twelve [12] rats weighing between 100 – 190g. In the first phase, four rats were divided into two groups of

Acute Toxicity and Lethality of Gladiolous psittacinus

Determination of the median lethal dose (LD50) of the plant extract was done in two phases. In the first phase, nine rats were divided into three groups of three rats each and were treated with the methanol extract of the plant at dosages of 10, 100 and 1000mg/kg body weight orally. They were observed for 24 hours for signs of toxicity.

Phytochemical and Anticonvulsant Properties of Annona ...

% of the seizures by the plant extract was 16 min at the dose of 150 mg/kg against 14 min for standard diazepam (2 mg/kg). In preventive mode, the plant extract prolonged the latency of convulsions. ... Determination of acute toxicity: The median lethal dose (LD50) ... lethal dose and the highest nonlethal dose from the second stage of dosing.

Sapium ellipticum (Hochst.) Pax Leaf Extract: In-vitro ...

Pax Leaf Extract: In-vitro Antioxidant Activities and Lethal Dose (LD 50) Determination in Wistar Rats O. M. Ighodaro 1,2* and O. A. Akinloye 1 1 Department of Biochemistry, College of Natural Sciences, Federal University of Agriculture, Abeokuta (FUNAAB), Abeokuta, Nigeria.

determination of lethal dose of plant extract

determination of lethal dose of plant extract. determination of lethal dose of plant extract. Determining LD50 for a plant extract Determining LD50 for a plant extract . What is the basis for selecting the minimal dose of plant extract for calculating lethal. Contact the manufacturer -->

Toxicity study of aqueous leaf extracts of Sarcocephalus ...

with an initial test dose to determine the approximate lethal and non-lethal doses of the extract according to method of (Turner, 1965). Twelve (12) rats of either were divided into four groups of three (3) rats per group. The animals were divided into a control group and three treatment groups each group consisting of three rats.

determination of lethal dose of plant extract

Determination of Lethal Doses of Volatile and Fixed Oils of ... Determination of lethal doses of several plants ... Lethal doses (mL/kg) Plant extract LD1 LD10 LD50 LD ... The lethal dose …

Chapter IV. Guidelines for Toxicity Tests

test) are designed to determine the mean lethal dose of the test substance. The ... Deichmann, W.B. and LeBlanc, T.J. (1943) Determination of the approximate lethal dose with about six